The rpoD gene functions as a multicopy suppressor for mutations in the chaperones, CbpA, DnaJ and DnaK, in Escherichia coli
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منابع مشابه
The r-poll gene functions as a multicopy suppressor for mutations in the chaperones, CbpA, DnaJ and DnaK, in Escherichia coli
The CbpA protein is an analog of the DnaJ molecular chaperone of Escherichiu coli. The &I./cbpAdouble-null mutant exhibits severe defects in cell growth, namely, a very narrow temperature range for growth. To gain insight into the functions of CbpA as well as DnaJ, we isolated a multicopy suppressor gene that permits this &XLcbpA _ mutant to grow normally at low temperatures. The suppressor gen...
متن کاملThe Escherichia coli DjlA and CbpA proteins can substitute for DnaJ in DnaK-mediated protein disaggregation.
The DnaJ (Hsp40) protein of Escherichia coli serves as a cochaperone of DnaK (Hsp70), whose activity is involved in protein folding, protein targeting for degradation, and rescue of proteins from aggregates. Two other E. coli proteins, CbpA and DjlA, which exhibit homology with DnaJ, are known to interact with DnaK and to stimulate its chaperone activity. Although it has been shown that in dnaJ...
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15 صفحه اولCbpA, a DnaJ homolog, is a DnaK co-chaperone, and its activity is modulated by CbpM.
The DnaK chaperone system, consisting of DnaK, DnaJ, and GrpE, remodels and refolds proteins during both normal growth and stress conditions. CbpA, one of several DnaJ analogs in Escherichia coli, is known to function as a multicopy suppressor for dnaJ mutations and to bind nonspecifically to DNA and preferentially to curved DNA. We found that CbpA functions as a DnaJ-like co-chaperone in vitro...
متن کاملInfluence of DnaK and DnaJ chaperones on Escherichia coli membrane lipid composition.
The content of fatty acids extracted from the membranes of E. coli MC 1061 harboring the wild-type dnaKdnaJ alleles and its delta dnaJ and delta dnaKdnaJ derivatives was compared. It was demonstrated that dodecanoic acid was missing in NPLs fraction extracted from both mutants grown at 42 degrees C. Phospholipids extracted from mutant strains were deprived of hexadecanoic acid methyl ester and ...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1996
ISSN: 0378-1097
DOI: 10.1016/0378-1097(96)00118-8